Bioluminescent and fluorometric techniques for determinations of 19 metabolites of ADP/ATP-dependent transformations in energy metabolism in 200 (or 400) mg muscle.

A procedure is described for the determination of the following 19 metabolites in 200 mg gastrocnemius muscle from guinea pig: glucose 1-phosphate and 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, 3-phospho- and 2-phosphoglyceric acid, phosphoenolpyruvate, pyruvate, glycerol, glycerol 3-phosphate, AMP, ADP, ATP, inorganic phosphate, creatine, creatine phosphate. Bioluminometric and fluorometric techniques for mono- and dinucleotide determinations were used. In the case of fluorometric measurement for NADH, 400 mg tissue were necessary. The coefficient of variation for assays on the same sample was 0.04 for bioluminometric techniques and 0.10 for fluorometric techniques.